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kod dna polymerase  (Toyobo)


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    Structured Review

    Toyobo kod dna polymerase
    Kod Dna Polymerase, supplied by Toyobo, used in various techniques. Bioz Stars score: 96/100, based on 2936 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kod+dna+polymerase/KOD+DNA+Polymerase/pm42888470-59-31-34
    Average 96 stars, based on 2936 article reviews
    kod dna polymerase - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Amplification:

    Article Title: A Prey–Bacterivore Interaction: CdtB Involvement in the Cytotoxicity Mechanism of Escherichia albertii Against Tetrahymena thermophila CU427
    Article Snippet: .. DNA Recombination The targeted region of cdtB of E. albertii was amplified using KOD DNA Polymerase (Toyobo) with CdtB for pCold primers (Table 1) and ligated into Sal1-cut pCold using In-Fusion cloning enzyme (Takara Bio). ..

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Cloning:

    Article Title: A Prey–Bacterivore Interaction: CdtB Involvement in the Cytotoxicity Mechanism of Escherichia albertii Against Tetrahymena thermophila CU427
    Article Snippet: .. DNA Recombination The targeted region of cdtB of E. albertii was amplified using KOD DNA Polymerase (Toyobo) with CdtB for pCold primers (Table 1) and ligated into Sal1-cut pCold using In-Fusion cloning enzyme (Takara Bio). ..

    Molecular Weight:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Marker:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Lysis:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Real-time Polymerase Chain Reaction:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Polymerase Chain Reaction:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Magnetic Beads:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    CCK-8 Assay:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Protease Inhibitor:

    Article Title: IFN-I induced-LAP3 promotes embryo resorption by inhibiting trophoblast mitophagy via targeting HSD17B10/PE pathway
    Article Snippet: Alexa Fluor Plus 647-conjugated goat anti-rabbit IgG (Invitrogen, A32728; 1:400), Alexa Fluor 488-conjugated goat anti-rabbit IgG (Invitrogen, A-11008; 1:400), and Alexa Fluor 633-conjugated streptavidin (Invitrogen, S21375; 1:400) were used for fluorescence detection. .. The following reagents were used: mdivi-1 (MedChemExpress, HY-15886), carbonyl cyanide 3-chlorophenylhydrazone (CCCP; MedChemExpress, HY-100941), chloroquine (Macklin, C843545), protein molecular weight marker (Epizyme Biomedical Technology, WJ103L), duramycin–LC–biotin (Mtarget, D-1003), Lα-phosphatidylethanolamine (L-α-PE; Sigma-Aldrich, P7943), Cell Lysis Buffer (CST, 9803S), ReverTra Ace qPCR RT Kit (TOYOBO, FSQ-101), 2× Hieff PCR Master Mix with Dye (Yeasen, 10102ES08), Lipofectamine 3000 (Thermo Fisher Scientific, L3,000015), anti-Flag magnetic beads (Invitrogen, A36797), anti-Myc magnetic beads (Invitrogen, 88842), protein A/G magnetic beads (Invitrogen, 88802), puromycin (Sigma-Aldrich, P8833), MitoTracker Orange (Invitrogen, M7510), Cell Counting Kit-8 (Focus, FCK-108), TRIzol LS Reagent (Invitrogen, 15596018CN), KOD DNA polymerase (TOYOBO, KOD-101), DAPI (Beyotime, P0131), poly(I:C) (Sigma-Aldrich, P1530), protease inhibitor cocktail (BBI Life Sciences, C600380), phosphatase inhibitor cocktail (BBI Life Sciences, C500017), and penicillin–streptomycin (Gibco, 15140122). ..

    Construct:

    Article Title: Identification of small-molecule inhibitors of the immune system repressor Regnase-1
    Article Snippet: .. All constructs were generated using KOD DNA polymerase (Toyobo) and In-Fusion (Takara) and verified by DNA sequencing. .. Plasmids were transformed into bacteria Lemo21(DE3) (NEB).

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Generated:

    Article Title: Identification of small-molecule inhibitors of the immune system repressor Regnase-1
    Article Snippet: .. All constructs were generated using KOD DNA polymerase (Toyobo) and In-Fusion (Takara) and verified by DNA sequencing. .. Plasmids were transformed into bacteria Lemo21(DE3) (NEB).

    DNA Sequencing:

    Article Title: Identification of small-molecule inhibitors of the immune system repressor Regnase-1
    Article Snippet: .. All constructs were generated using KOD DNA polymerase (Toyobo) and In-Fusion (Takara) and verified by DNA sequencing. .. Plasmids were transformed into bacteria Lemo21(DE3) (NEB).

    Transgenic Assay:

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Plasmid Preparation:

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Transformation Assay:

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Over Expression:

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China

    Sequencing:

    Article Title: PuWRKY46 drives root system architecture and enhances salt tolerance in poplar.
    Article Snippet: Haoqin Zhao (赵浩钦) , Shicheng Zhao (赵世成) , Tiantian Sun (孙甜甜) , Wenhui Zhuang (庄文慧) , Jinhui Gao (高金辉) , Houliang Zhang (张厚良) , Jingru Ren (任婧茹) , Qianqian Liu (刘倩倩) and Jingli Yang (杨静莉) State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, 150000, China; School of Pharmacy, Harbin University of Commerce, Harbin, 150000, China; Yichun Branch of Heilongjiang Academy of Forestry, Yichun, 153000, China



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